NUTRITION, GENETICS AND BIOTECHNOLOGY DIVISION

PROFILE

Nutrition Section

Nutrition, Genetics and Biotechnology Division of Central Institute of Brackishwater Aquaculture is the pioneer in Aquaculture Nutrition research in India. The focussed research programmes implemented by the division led to the development and commercialisation of several feed technologies in the country to benefit small and medium scale farmers. Feed being the major and critical input in aquaculture, considerable thrust has been given to feed development programmes. Feed for brackishwater species have undergone considerable changes over the past 25 years at this institute from the use of trash fish or live fish, to a simple pellet-pressed moist diet to a modern, high-nutrient dense pellet. The state of the art facilities available at the institute with the collaboration of private entrepreneur/corporate on a Public Private Partnership (PPP) mode has contributed substantially to sustainable brackishwater aquaculture. The Indigenous Shrimp Feed Technology for tiger shrimp has been developed by conducting series of feeding trials under controlled laboratory conditions followed by on-station and on-farm trials in farmer’s ponds. This technology was commercialised to M/S Bismi Feeds (P) Ltd. CIBA has also developed a low fish-meal shrimp feed to improve the cost effectiveness and sustainability. This led to the development of Organic Shrimp feed (Green Shrimp BT) and the CIBA feed mill has been certified to produce Organic feed. The significant contribution of the division is the development of cost-effective shrimp feed for Penaeus vannamei branded as vannamei Plus. The field demonstration in various multi-locational trials demonstrated the cost effectiveness of this feed and it has become the most sought after feed. This technology was commercialised in the states of Andhra Pradesh, Kerala, Gujarat and West Bengal on a non-exclusive basis. CIBA also has developed the cost effective feed for Asian Seabass, grow-out culture, Seabass Plus.

CIBA possesses the state-of-art facilities to prepare different grades of sinking and floating pellets. Larval feeds (100 µ to 500 µ) and broodstock feeds for both Shrimp and Finfish have been developed. The modern feed processing machineries like ultra-fine grinder, Ring die pelletizer, Extruder, Spheronizer, vaccum coater and Spray dryer are effectively utilised to prepare the larval, broodstock and functional feeds. The Division is having a fully equipped analytical and quality control laboratory with facilities for analysis of major and minor nutrients. A modern wet laboratory to carry out feeding experiments in different life stages of shrimp and fish is the additional forte to this division. A micro algal laboratory for screening, isolation, identification and culture of different microalgae is the recent addition to the list.

The division is mandated to develop cost-effective environmentally sustainable feeds for all the brackishwater candidate species, and also to develop functional feeds for broodstock, larvae and specialty feeds to address the niche markets.

Genetics & Biotechnology Section

Since inception in 2004, the Genetics and Biotechnology Section of the division focuses on genetic characterization of candidate aquaculture species, development and utility of markers and genes for improving economic traits. This division has scientists belonging to different relevant disciplines of Genetics, Biotechnology and Aquaculture. The GBS has well established laboratory facilities and expertise for carrying out cutting edge research in the niche areas of molecular biology and genetics to conduct genetic improvement programs in fish and shrimp species. Recent developments in biotechnology and information technology have paved the way for deeper understanding of the molecular mechanisms involved in physiology, reproduction and diseases. Further, basic understanding in molecular mechanisms in physiology will help in technological interventions in reproductive biology and culture practices. The unit has documented information on expression of genes that is useful for comprehending shrimp response to biotic and abiotic stresses and also those that help in inducing breeding in shrimp. In collaboration with the Norwegian Institute of Food, Fishery and Aquaculture (NOFIMA), Norway, the section has constructed for the first time, a SNP-based linkage map and a DNA chip containing SNPs useful for genome-wide association studies in tiger shrimp. The section is well-equipped with hardware necessary for conducting bioinformatics analyses with high-through put data and is gradually building up the expertise in the field.

Partnership and linkages GBS has developed advanced research facilities over the years with funding support from ICAR, National Agricultural Innovation Project (NAIP), Department of Biotechnology (DBT) and Consortia Research Platform on Genomics. Several International collaborations have been developed under the externally funded projects in UK with University of Southampton, University of Aberdeen, Swansea University, University of St. Andrews, University of Liverpool and Bangladesh Agricultural University, Bangladesh. There was also a collaboration between this institute, NBFGR and Temasek Life Sciences Laboratory (TLL), Singapore on the genetic characterisation of Asian seabass Lates calcarifer. At the National level, research collaborations have been undertaken with IIT, University of Madras and Anna University, Chennai. Efforts are on to have research collaboration with the Tamil Nadu Dr. J. Jayalalitha Fisheries University at Nagapattinam.

GBS mandate The GBS is mandated to carry out research on
  1. Genetics and Breeding in aquatic animals aiming at genetic improvement
  2. unravelling host pathogen interaction at the molecular level
  3. Genomics and transcriptomics
  4. Bioinformatics.

SALIENT ACHIEVEMENTS

Nutrition Section

The nutrition section of ICAR- Central Institute of Brackishwater Aquaculture has developed the following viable cost effective feed technologies for brackishwater aquaculture. Feed being a critical input in aquaculture, Aquafeed formulation and manufacturing become complex when we consider the economics, environmental sustainability, nutritional adequacy and constrains in feed manufacturing and storage. Thus, the way in which feed ingredients are carefully chosen, processed, mixed, and prepared is controlled by many elements, and compromise between the ideal and the practical is a necessary for successful production of compounded aquafeed.

1.Compressed dry pellet feed processing technology with indigenous machineries Earlier in 90’s Indian shrimp farming sector was completely dependent on imported feeds. No single public or private entity was having the technology to manufacture pelleted sinking feed for shrimp and other fishes. Visualizing this as a critical obstacle, ICAR-CIBA developed, tested and commercialized an indigenous feed manufacturing technology in India. This kind of pelleting technology is useful in making feeds for shrimp, crab, grow-out and brood fishes.

Highlights of the expertise/technology: Impact of the technology:

2. Extruded floating and slow sinking feed processing technology Cooking extrusion is the most recent development in pelleted fish feed manufacture. In this process a set of mixed ingredients are forced through an opening in a perforated plate or die with a design specific to the food, and is then cut to a specified size by swiftly rotating blades. The extruder machine functions by increasing the temperature of the feed mixture to 125–150oC in a pressurized conditioning chamber and increasing the moisture by more than 20%. Extruded pellets are relatively porous and can soak up sprayed oil to reach levels of over 20%, typical of feed for both brood and grow-out feeds of Mullet, Milkfish and Seabass.

Highlights of the expertise/technology:

3. Larval feed processing technology involving spray drying, extrusion cooking, spheronization and freeze drying CIBA is involved in development of seed and feed for diversified brackishwater aquaculture species, and has developed captive breeding of several finfish and shellfish of different trophic nature. Optimum nutrition is the key driver to maximise the reproductive performance and seed yield. Larval feeds are specialty feeds, which is one of the vital elements for a successful hatchery operation. At present 100% of the larval feeds used in Indian shrimp hatcheries are imported, and are very expensive. Visualizing the demand for the larval feeds, CIBA has developed feeds for different larval stages. Micro-bound and micro-particulate feeds of different particle sizes (300 to 800 micron) were prepared. Advanced micro bound and micro particulate larval feeds for shrimp larvae produced with the state of the art facilities such as spray drying, extrusion cooking, spheronization and freeze drying available at CIBA’s larval feed production unit.

Highlights of the larval feed technology:

 Demonstration of Vacuum coater to the RAC team

4. Farm made feed processing technology As a next step after success in captive breeding of brackishwater finfishes CIBA is promoting farming of finfishes as alternate for shrimp. Feed is the critical input which directly determines the growth performance of the farmed candidate and profitability of the aquaculture. India being agriculture based economy and resources, there are plenty of region specific agriculture and animal by-products available for potential use in feed formulation. ICAR-CIBA conceived a concept called farm made feed technology to produce on site low cost feeds for farmed brackishwater fishes.

  Highlights of this farm made feed technology:

Products developed: Cost effective indigenous feeds for brackishwater Aquaculture

1. Indigenous cost effective vannamei shrimp feed: VanamiPlus Size of the Indian shrimp feed industry is 6.25 lakh tonnes worth Rs 4600 crores in year 2016. Increasing shrimp feed price is a major challenge facing Indian shrimp farming. ICAR-CIBA took strategic effort and developed a cost effective feed, VanamiPlus in identifying the untapped feed resources across the country and organized balanced shrimp feed formulas using those resources considering the optimum nutrient requirements of the shrimp and feed processing needs. Features of the technology: VanamiPlus

Impacts of the VanamiPlus:

[caption id="attachment_1543" align="alignleft" width="300"] Commercial Launch of Desi, Cost Effective Shrimp Feed (VanamiPlus)[/caption] [caption id="attachment_1544" align="alignleft" width="300"] Shrimp Harvest with VanamiPlus in Kerala[/caption] [caption id="attachment_1546" align="alignleft" width="300"] Shrimp Harvest with VanamiPlus in Punjab[/caption] [caption id="attachment_1547" align="alignleft" width="300"] Feed mill established in Bapatla, A.P. with CIBA Technical Colloboration[/caption] [caption id="attachment_1554" align="alignleft" width="300"] Feed Packing at Bapatla Feed Mill[/caption] [caption id="attachment_1555" align="alignleft" width="300"] MoU signing for establishment of Shrimp feed mill in Haryana – First of its kind in North India[/caption]
   

2. Organic Shrimp feed: Organic concept has been widely adopted in aquaculture, and critical input is the organic certified feed. Organic feed production is the holistic method of feed

production following the organic principles. In country like India it has lot of scope, where major portion of farmed shrimp is exported. There is a huge demand for organically farmed premium shrimp in American and Japanese market. Highlights of the product developed:

Process of the product Development:

Demonstration and Validation of the technology:

3. EtroBroodPlus – Functional broodstock feed for pearlspot to yield higher fry production under captive conditions Termination of parental care was a key element in inducing the pearlspot for multiple spawning. Repeated spawning by same pair needs adequate functional nutrition to yield higher number healthy fry. Considering the nutritional requirements of pearlspot, ICAR- CIBA developed, a functional feed “EtroBroodPlus” for sustaining the higher fry yield.

Highlights of the EtroBroodPlus: Pearlspot Feed demonstration in Kerala:  

4. SeebassPlus –A feed for growout culture of seabass Asian sea bass is a high value, fast growing food fish, which is highly carnivorous and predatory in nature. ICAR-CIBA identified sea bass as a candidate fish for farming in brackishwater for diversification of farming. Feeding such a carnivore sea bass is challenging in its farming, in terms of feed acceptance as well as feed cost. As outcome of continuous research, CIBA developed a cost effective feed for sea bass and branded it as “SeebassPlus”.

            Features SeebassPlus: 5. Milkfish Brood Plus – A feed for Milkfish broodstock Milk is a fast-growing herbivorous finfish suitable for farming in brackishwater ponds. Spawning adults will be 5 to 15 kg and need quality broodstock feed for realizing the maximum reproductive potential under captive conditions. Considering its feeding biology, it needs a specialty feed with unique formula to meet its reproductively important fatty acids and micro nutrients. Considering this chalenges and significance, CIBA developed a broodstock feed and tested with our own milkfish breeding program, which got a success in breeding milkfish in 2015 for first time in this country. The specialty feed for milkfish broodstock has been branded as Milkfish BroodPlus. Features of Milkfish BroodPlus:   6. Seebass NurseryPlus – A feed for nursery culture of seabass

Seabass larvae and juveniles are highly cannibalistic in the absence of suitable feeds, therefore apt feed and proper weaning are key elements in viable seed production of seabass. In this setting, CIBA developed micro bound nurserry feeds for seabass using extrusion cooking. Feeds were formulated to contain 55% crude protein and 15% li

pid, using premium ingredients of high digestibility and nutritive value.

Features of Seebass NurseryPlus:

7. Milkfish NurseryPlus – A nursery feed for intensive nursery rearing of Milkfish    

For the first time in this country, artificial breeding of milkfish under captive conditions was achieved on 2015, at our Muttukkadu fish hatchery. Getting appropriated size milkfish seed is a bottleneck in large scale farming of its large scale farming. Larval and nursery rearing with suitable feed and rearing system is the next critical step, to scale up seed production and commercialize the milkfish production. We developed micro particulate dry feeds and feeding strategy for mass rearing of milkfish fry in outdoor green water system. Micro particulate larval feeds were formulated and prepared by cold extrusion and spheronization to contain 42% protein and 8% lipid.

8. MilkfishPlus –A grow out feed for Milkfish Milkfish is a fast-growing herbivorous finfish suitable for farming in brackishwater ponds. To back up its rapid growth in brackishwater ponds and ensure profits to the farmer, a cost-effective feed is mandatory. In this angle, CIBA developed a cost-effective feed wit locally available feed ingredient, mostly of plant ingredients. The feed was extensively tested in farmers ponds for growth performance and economics. This feed technology is ready for commercialization.

Highlights of the MilkfishPlus:

9. PolyPlus – A grow out feed for polyculture of fish and shrimp

ICAR-CIBA promotes polyculture of brackishwater species to have better utilization of resources. At present farmers practise the polyfarming in tide fed ponds. They normally do not use any feed. However, few farmers use mash feed (mixture of mustard oil cake and rice bran). Availability of low cost pellet feed is a bottle neck to harness the growth potential of brackishwater polyfarming. Considering this situation, the ICAR-Central Institute of Brackishwater Aquaculture (ICAR-CIBA) -initiated development of low cost feed for multispecies culture using locally available ingredients as partial replacement of the most expensive feed ingredient, fish meal.

Features of PolyPlus:

10. Shrimp LarviPlus A functional feed for Shrimp post larvae Functional feeds are formulated to have different biologically active compounds that can provide specific benefits to the farmed shrimp, in addition to just nutritional. The ingredients with such known functional properties are called functional ingredients. Functional feeds may be targeted for to any specific function related to reproduction, larval development, physiology, immunity and gut health. Larval feeds are specialty feeds, which is one of the vital elements for a successful hatchery operation. At present 100% of the larval feeds used in Indian shrimp hatcheries are imported, and are very expensive. Visualizing the demand for the larval feeds, CIBA has developed feeds for different larval stages.

Highlights of Shrimp LarviPlus:

11. CyllaPlus-– A grow out feed for the Mud crab

Mud crab is an high value seafood commodity farmed in brackishwaters. Both farming and fattening of crab still mostly depend on conventional unsustainable feeds such as trash fish, bivalve meat and other meaty foods, and sourcing of these foods is always a constrain. CIBA developed two different types of feeds for crab, one each for grow-out (Cylla GPlus) and fattening (Cylla FPlus) using indigenous ingredients to contain 35% protein and 5.6% fat.

Features of crab feed:

12.HilsaPlus-– A grow out feed for Hilsa (Tenualosa ilisha)

Tenualosa ilisha, commonly known as Hilsa, has been one of the most important fish species in south Asia in general and West Bengal in particular. Although captive breeding has been attempted since 1900, the successful closing of the life cycle has been still enigmatic. Captive spawning of hilsa using wild caught broodstock was standardized and embryonic developmental stages of fertilized egg were studied under microscope at different hours of incubation in Kakdwip Research Centre of ICAR-CIBA. Further, Nutrient requirement of hilsa fry were established through indoor trial. Considering the protein, lipid and amino acid requirement, formulated feed (Hilsaplus) with 35 % protein and 12 % lipid has been developed for hilsa grow-out culture. Wild caught hilsa seeds attained 350-425 g after two and half years of culture with formulated feed, Hilsaplus in brackish water ponds.

Features of Hilsa Plus:

13.Design and development of low cost indigenous solar powered automatic feeder for L. vannamei

The major component of scientific shrimp farming is to provide pelleted feeds at right quantity at right time according to the requirement. With the introduction of P. vannamei culture since 2009, automatic feeders have become a necessity as more stocking density is being adopted. Automatic feeders are more suitable for P. vannamei culture due to its feeding behaviour as it is a column dweller and it needs continuous feeding. Entrepreneur farmers have imported automatic feeders and it is costly. These prompted to design and develop a low cost feeder for the use for small farmers. Development of the automatic feeder / solar powered automatic feeder:

         

SCIENTISTS

 
Dr.M.Shashi Shekhar, Principal Scientist & Scientist-In-Charge (SIC) Email:MS.Shekhar@icar.org.in
Phone : +91- 044 - 24618817, Ext - 301
Dr.K.Ambasankar, Principal Scientist & In-charge of Nutrition Group Dr.J.Syama Dayal, Principal Scientist Dr. T.K.Ghoshal, Principal Scientist Dr. Debasis De, Principal Scientist & OIC, KRC Shri. J. Ashok Kumar, Scientist Dr. K.P. Kumaraguru Vasagam, Principal Scientist  & OIC, PME Dr.Ms. Sherly Tomy, Principal Scientist Dr.Sivamani Balasubramaniam, Senior Scientist Dr.K.Vinaya Kumar, Scientist Shri. Sandeep,K.P, Scientist Mr. Sivaramakrishnan T., Scientist Dr.J. Raymond Jani Angel, Scientist Ms. Misha Soman, Scientist Ms. Leesa Priyadarsani, Scientist